Lifeboat Foundation

Chromatin proteins have expanded the mammalian synthetic biology toolbox by enabling control of active and silenced states at endogenous genes. Others have reported synthetic proteins that bind DNA and regulate genes by altering chromatin marks, such as histone modifications. Previously we reported the first synthetic transcriptional activator, the “Polycomb-based transcription factor” (PcTF), that reads histone modifications through a protein-protein interaction between the PCD motif and trimethylated lysine 27 of histone H3 (H3K27me3). Here, we describe the genome-wide behavior of PcTF. Transcriptome and chromatin profiling revealed PcTF-sensitive promoter regions marked by proximal PcTF and distal H3K27me3 binding. These results illuminate a mechanism in which PcTF interactions bridge epigenetic marks with the transcription initiation complex. In three cancer-derived human cell lines tested here, many PcTF-sensitive genes encode developmental regulators and tumor suppressors. Thus, PcTF represents a powerful new fusion-protein-based method for cancer research and treatment where silencing marks are translated into direct gene activation.

OAHU, HAWAI’I —(ENEWSPF)–September 1, 2016. As thousands of government representatives and conservationists convene in Oahu this week for the 2016 World Conservation Congress, international conservation and environmental leaders are raising awareness about the potentially dangerous use of gene drives — a controversial new synthetic biology technology intended to deliberately cause targeted species to become extinct.

Members of the International Union for the Conservation of Nature (IUCN), including NGOs, government representatives, and scientific and academic institutions, overwhelmingly voted to adopt a de facto moratorium on supporting or endorsing research into gene drives for conservation or other purposes until the IUCN has fully assessed their impacts. News of the August 26 digital vote comes as an important open letter to the group is being delivered.

Scientists and environmental experts and organizations from around the globe have advocated for a halt to proposals for the use of gene drive technologies in conservation. Announced today, a long list of environmental leaders, including Dr. Jane Goodall, DBE, genetics professor and broadcaster Dr. David Suzuki, Dr. Fritjof Capra, entomologist Dr. Angelika Hilbeck, Indian environmental activist Dr. Vandana Shiva and organic pioneer and biologist Nell Newman, have lent their support to the open letter: “A Call for Conservation with a Conscience: No Place for Gene Drives in Conservation.” The letter states, in part: “Gene drives, which have not been tested for unintended consequences, nor fully evaluated for ethical and social impacts, should not be promoted as conservation tools.”

“Fifty years after the show aired, Star Trek’s fictional tricorder is far from becoming a reality. But a $10 million prize from the XPRIZE Foundation is hoping to motivate inventors to create one quickly.”

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Cancer thrives when mutated cells undergo frequent division. Most anti-cancer drugs work by inserting themselves in between the DNA base pairs that encode our genetic information. This process is known as intercalation, and it can result in subtle changes to the DNA molecule’s geometric shape or tertiary structure. These structural changes interfere with the DNA’s transcription and a cell’s replication process, ultimately resulting in cell death.

While intercalating agents used in chemotherapy drugs are highly effective in fighting cancer, they also may kill important cells in the body and lead to other complications such as heart failure. Therefore, researchers are always searching for faster, cheaper and more accurate tools to aid in the design of next-generation anti-cancer drugs with reduced side effects.

A paper published in ACS Nano, one of the top nanotechnology journals in the world, explores this topic. “Modeling and Analysis of Intercalant Effects on Circular DNA Conformation,” (LINK TO http://pubs.acs.org/doi/abs/10.1021/acsnano.6b04876) focuses on the effect of the intercalating agent ethidium bromide (a mimic for many chemotherapy drugs) on the tertiary structure of DNA.

Continue reading “Solid-State Nanopores Unravel Twisted DNA Mystery” »

Glad this discovery has been found; however, sad to hear as well. Sharing for my friends involved with anti-aging (Alex) and others work on the cancer cure.

Genetic signature of the brain cancer cell lines used for research is different from the original patient tumor cells.

Experts may reassure us that artificial intelligence won’t take over the world anytime soon – but they just might invade the multiplex.

At least that’s the plot developing at IBM, where the Watson artificial-intelligence team programmed a computer to come up with a scary trailer for “Morgan,” a thriller about a genetically modified, AI-enhanced super-human.

Researchers genetically modify piglets to be resistant to an incurable disease plaguing hog farms, Porcine Reproductive Respiratory Syndrome (PRSS). The researchers cut out a specific gene to cure the pigs.

It’s an add-on for CRISPR.

Researchers have created a new genome editing technique called Target-AID, which induces point mutations instead of cutting DNA

Gene editing technology has fantastic potential, but there are remaining issues and questions over safety and specificity. The major contender is currently CRISPR-Cas9, but this induces a double stranded break in DNA which is a slightly riskier approach — particularly if it cuts in other locations too that you don’t want it to. Research teams across the world are both optimising and customising the CRISPR system; creating more accurate versions or versions that regulate gene expression as opposed to editing it. One such team has now built an add-on to CRISPR, Target-AID.

Continue reading “Safer Gene Editing Without Cleaving DNA” »

From selective breeding to genetic modification, our understanding of biology is now merging with the principles of engineering to bring us synthetic biology.

Written, animated and directed by James Hutson, Bridge8.

Continue reading “Synthetic Biology Explained” »