Breaking the bacteria barriers.
If that field is at just the right magnitude, it will open up pores within the cell membrane, through which DNA can flow. But it can take scientists months or even years to figure out the exact electric field conditions to reversibly unlock a membrane’s pores.
A new microfluidic device developed by MIT engineers may help scientists quickly home in on the electric field “sweet spot” — the range of electric potentials that will harmlessly and temporarily open up membrane pores to let DNA in. In principle, the simple device could be used on any microorganism or cell, significantly speeding up the first step in genetic engineering.
“We’re trying to reduce the amount of experimentation that’s needed,” said Cullen Buie, the Esther and Harold E. Edgerton Associate Professor of mechanical engineering at MIT. “Our big vision for this device and future iterations is to be able to take a process that usually takes months or years, and do it in a day or two.”
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